Document Type : Research Paper


Department of Biology, Dezful Branch, Islamic Azad University, Dezful, Iran.


In order to investigate the biofilm creating bacteria in drinking water of Ahvaz, Iran, 4 different types of frequently used kitchen dishes (made up of plastic, glass, zinc, and teflon) were used for storage of drinking water under the same conditions at room temperature (25 °C) in triplicate order. After the formation of the slime layer, microbiological tests were performed. Results showed that after 3 days, the biofilm layer was created. The biofilm creating bacteria of studied water belonged to both the gram-negative and gram-positive groups and were identified as follows: Staphylococcus aureus, Eschershia coli, Salmonella typhimorium, Entrobacter aerogenes, and Shigella sp. Results showed that the plastic dishes had the highest rate of bacterial growth and E. coli with 65 % of the growth was the most abundant bacteria of the investigated biofilm. It could be concluded that even in purified drinking water there were bacteria with the ability to create biofilm which needs more attention to purification processes and water storage in consideration with the quality of the dishes.


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