Document Type : Research Paper
Authors
Department of Biology, Dezful Branch, Islamic Azad University, Dezful, Iran.
Abstract
In order to investigate the biofilm creating bacteria in drinking water of Ahvaz, Iran, 4 different types of frequently used kitchen dishes (made up of plastic, glass, zinc, and teflon) were used for storage of drinking water under the same conditions at room temperature (25 °C) in triplicate order. After the formation of the slime layer, microbiological tests were performed. Results showed that after 3 days, the biofilm layer was created. The biofilm creating bacteria of studied water belonged to both the gram-negative and gram-positive groups and were identified as follows: Staphylococcus aureus, Eschershia coli, Salmonella typhimorium, Entrobacter aerogenes, and Shigella sp. Results showed that the plastic dishes had the highest rate of bacterial growth and E. coli with 65 % of the growth was the most abundant bacteria of the investigated biofilm. It could be concluded that even in purified drinking water there were bacteria with the ability to create biofilm which needs more attention to purification processes and water storage in consideration with the quality of the dishes.
Keywords
Boe-Hansen R., Martiny A.C., Arvin E., Albrechtsen H.J., Monitoring biofilm formation and activity in drinking water distribution networks under oligotrophic conditions, Water Science and Technology 47 (2003) 91-97.
Bomo A.M., Storey M.V., Ashbolt N.J., Detection, integration and persistence of aeromonads in water distribution pipe biofilms, Journal of Water and Health 2 (2004) 83-96.
Characklis W.G., McFeters G.A., Marshall K.C., Physiological ecology in biofilm systems, First Ed., John Wiley & Sons, New York; (1990).
Costerton, J., Geesey G., Cheng K., How bacteria stick, Scientific American 238 (1987) 86-5.
Flemming H., Biofouling in water system- cases, Causes and countermea sures, Applied Microbiology and Biotechnology 59 (2002) 629-40.
Juhna T., Birzniece D., Larsson S., Zulenkovs D., Shapiro A., Azevedo N.F., Menard-Szczebara F., Castagnet S., Feliers C., Keevil C.W., Detection of Escherichia coli in biofilms from pipe samples and coupons in drinking water distribution networks, Applied Environmental Microbiology 73 (2007) 7456-7464.
Mirhendi S.H., and Nikaeen M., Wastewater microbiology, First Ed.,Tehran University of Medical Sciences, Iran; (2004) (In Persian).
Percival S., Knapp J.S., Edyrean R., Wales D., Biofilms, mains water and stainless steel, Water Research 32 (1998) 2187-201.
Rivardo F., Turner R., Allegrone G., Ceri H., Martinotti M., Antiadhesion activity of two biosurfactants produced by Bacillus spp. prevents biofilm formation of human bacterial pathogens, Journal of Applied Microbiology and Biotechnology83 (2009) 541-553.
September S., Els F., Venter S., Brozel V., Prevalence of bacterial pathogens in biofilms of drinking water distribution systems, Journal of water Health 5 (2007) 219-227.
Szymanska J., Biofilm and dental unit water lines, Annual Agriculture and Environment Medicine 10 (2003) 151-57.
Yari A., Safdari M., Hadadian L., Chemical and physical and microbial quality evaluation of treated water by desalination in Qom during 2002, Qom University of Medical Sciences Journal 1 (2007) 45-54.
Zottola E.A., Microbial attachment and biofilm formation: A new problem for the food industry?,Food Technology 48 (1994) 107-114.